Auteurs :
Fabienne Loisy ^a, Robert L. Atmar ^b, Jean Cohen ^c, Albert Bosch ^d, Françoise S. Le Guyader ^a.

Journal :
Research in Microbiology, 2004, vol.155, 575-578.

Type :
Publication

Affiliation :

a Laboratoire de Microbiologie, IFREMER, BP 21105, 44311 Nantes cedex 3, France
b Department of Molecular Virology and Microbiology, Baylor College of Medicine, One Baylor Plaza, Houston, TX 77030, USA
c Unité mixte CNRS-INRA, Virologie Moléculaire et Structurale, 1 avenue de la Terrasse, 91198 Gif/Yvette, France
d Enteric Virus Group, Department of Microbiology, University of Barcelona, Diagonal 645, 08028 Barcelona, Spain

Résumé :
The potential of rotavirus 2/6-virus-like-particles (VLP2/6) for use as tracers in the marine environment was investigated. The stability of bovine rotavirus (strain RF) and VLP2/6 in natural seawater at 25degreesC for six days was studied. ELISA and western blot methods were used to quantify the particles. The rates of decline of rotavirus particles and VLP2/6 were similar (approximately 0.5 log 10 per day). Western blot analysis showed that the integrity of capsid proteins VP2 and VP6 was conserved during the incubation time. These results demonstrate that VLP2/6 particles have the same stability in seawater as rotavirus particles. Thus, VLP2/6 can be used as a tracer, which should be of particular value for studying the fate of rotavirus particles in the marine environment..

Mots Clés :
Rotavirus, Traceurs, Eau de Mer, Réaction en Chaîne par Polymérase, RT-PCR, Milieu Marin