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Genotyping Pseudomonas aeruginosa

Overview of P. aeruginosa

P. aeruginosa is an ubiquitous environmental bacterium and a human pathogen. Due to its large occurrence in hospital water systems and its faculty to persist on medical devices, P. aeruginosa is a leading cause of hospital-acquired pneumonia. To track the primary source of infection and the spread of P. aeruginosa in the health care facility, Ceeram has been developing a powerful typing method.

What is Bacterial Typing?

A bacterial typing method is a phenotypic or genetic analysis of bacterial isolates performed to generate isolate-specific fingerprints for assessing epidemiological relatedness. This analysis is based on polymorphic phenotypic or genetic biomarkers to differentiate each individual in the entire specie.

Why typing P. aeruginosa

Identify and trace outbreaks in a real time from a single or a multiple source.
Check if a medical device is the source of a contamination to implement hygiene measures.v
Distinguish recurrence and reinfection in a chronic patient to determine the source of infection.
Check if the hospital water network is the source of contamination to characterize the outbreak (nosocomial or community-acquired).
Show evidence of cross-infections between patients to control the outbreak.
Emphasize the emergence of virulent strains/clones (Lesb, PAO1, PA14,…)
Study P. aeruginosa epidemiology to know its evolution and evaluate emergence conditions.

MLVA, a powerful typing method

Multi Locus VNTR Analysis (MLVA) is a PCR based typing method that relies on the inherent variability found in many regions of repetitive DNA called VNTR (Variable Number Tandem Repeat) which represent sources of genetic polymorphism. The MLVA assay for P. aeruginosa examines 16 loci. Thus, each isolate is defined by a 16-digit numeric code corresponding to the number of repeats at each VNTR.

Genotyping Kit

Efficient amplification of the 16 markers in 2 multiplex PCRs. Required equipment: ABI sequencer to analyze the amplicons. The typing data is managed by GeneMapper (defaultly linked with ABI sequencers) to obtain the numeric code. Ceeram provides specific and adapted training sessions to help the implementation of bacterial typing in your lab.

Ceeram Solution : Service

Our Expertise Department has an automatized and standardized genotyping platform at disposal. The procedure involves three steps:

Samples receipt (DNA or colonies on plates)
MLVA genotyping and
Analysis report (isolates relatedness).

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Keywords : Typing P. aeruginosa, Typing Pseudomonas, Genotyping yeast, genotyping mould, genotyping bacteria