The polymerase chain reaction allows a specific region in a genome to be amplified and multiplied. There are various types of PCR, one of which is real-time PCR. Unlike “classical” PCR, which delivers final results that are visible on agar or acrylamide gel, real-time PCR uses a probe coupled with a fluorochrome specific to the sequence to be amplified. During each cycle, fluorochromes are released and the quantity of fluorescence emitted is proportional to the quantity of nucleic acids present.